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cDNA library: NIH_ZGC_35

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Information for 1 library

ovary

   1. NIH_ZGC_35

NIH_ZGC_35

Name: NIH_ZGC_35
Library ID: 2421
Organism: Danio rerio
Strain: AB
Gender: female
Age: 0
Stage: adult
Organ: ovary
Tissue: pooled ovaries, including ooctyes and somatic tissue
Host: DH10B TonA
Vector: pExpress-1
Vector type: plasmid
Insert digest: 5' EcoRV/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3', double-stranded cDNA was cloned into the NotI and EcoRV sites of pExpress-1 (EcoRV site destroyed upon ligation). Library was size-selected for an average insert size of 1.7 kb. Primary library, non-amplified. Use M13(-21) to generate 3' end reads and M13R to generate 5' end reads. Library was constructed by Open Biosystems (Huntsville, AL). Note: this is a Zebrafish Gene Collection library.

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