Name: WTSI-ohORF Library ID: 2423 Organism: Homo sapiens Age: 0 Organ: unspecified Host: Mach1 T1-resistant Vector: pENTR223 Vector type: Gateway entry vector Insert digest: 5' attL1.1/attL2.1 3' Stop Codon Status: without Description: ORFs flanked by att sites were amplified from fully sequence verified cDNA clones in two rounds of PCR. Amplified products were separated by agarose gel electrophoresis, excised, purified and recombined into pDONR223 using BP clonase TM. After full sequence verification, only clones which exactly matched the original cDNA and att sequences were accepted. Clone structure of the ORF and flanking sequences is as follows: 5'-gtacaaaaaagttgGCACC-ORF-ccaactttcttgtac-3' where lower case corresponds to the att sites and upper case corresponds to linker sequence. Clones from this library do not contain a stop codon. Library constructed by the Wellcome Trust Sanger Institute for the ORFeome Collaboration.
