Name: NICHD_XGC_tropLimb_m Library ID: 2426 Organism: Xenopus tropicalis Strain: TGA Age: 0 Stage: embryo Organ: limb Host: DH10B TonA Vector: pCS107 Vector type: plasmid Insert digest: 5' SalI/NotI 3' Stop Codon Status: without Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.