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cDNA library: NICHD_XGC_tropTe1

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Information for 1 library

testis

   1. NICHD_XGC_tropTe1

NICHD_XGC_tropTe1

Name: NICHD_XGC_tropTe1
Library ID: 2430
Organism: Xenopus tropicalis
Strain: TGA IC
Gender: male
Age: 0
Stage: adult
Organ: testis
Host: DH10B TonA
Vector: pCS107
Vector type: plasmid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.

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