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cDNA library: NIH_MGC_436

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Information for 1 library

synthesized DNA

   1. NIH_MGC_436

NIH_MGC_436

Name: NIH_MGC_436
Library ID: 2492
Organism: Mus musculus
Age: 0
Organ: synthesized DNA
Host: DH10B TonA
Vector: pENTR223.1
Vector type: Gateway entry vector
Insert digest: 5' attL1/attL2 3'
Stop Codon Status: with
Description: DNA synthesis was used to prepare the protein coding sequence (ORF) and flanking sequences, permitting directional cloning into the Gateway Entry vector, pENTR223.1 (recombinational cloning details are described in http://mgc.nci.nih.gov/Vectors/prot_pENTR223.1). Clone structure of the ORF and flanking sequences is as follows: 5'-gtacaaaaaagcagaagGGCCGTCAAGGCCCACC-ORF-TAAGGCCTCATGGgcccagctttcttg-3' where lower case corresponds to the att sites and upper case corresponds to linker sequence. Clones from this library do contain a stop codon. Clones were synthesized by Codon Devices (Cambridge, MA). Note: library donated to the ORFeome Collaboration by the Mammalian Gene Collection.

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