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cDNA library: NIH_ZGC_37

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Information for 1 library

mixed

   1. NIH_ZGC_37

NIH_ZGC_37

Name: NIH_ZGC_37
Library ID: 2498
Organism: Danio rerio
Age: 0
Stage: mixed
Organ: mixed
Tissue: whole embryo 5 somites, whole embryo 20 hpf, whole embryo 24 hpf, whole embryo 48 hpf, whole adult fish
Host: DH10B TonA
Vector: pCR4-TOPO
Vector type: plasmid
Insert digest: EcoRI
Stop Codon Status: without
Description: Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_XXX has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.

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