Name: NIH_ZGC_40 Library ID: 2501 Organism: Danio rerio Age: 0 Stage: mixed Organ: mixed Tissue: whole embryo 5 somites, whole embryo 20 hpf, whole embryo 24 hpf, whole embryo 48 hpf, whole adult fish Host: DH10B TonA Vector: pCR-XL-TOPO Vector type: plasmid Insert digest: EcoRI Stop Codon Status: without Description: Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_XXX has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.