Name: NIH_XGC_1 Library ID: 2514 Organism: Xenopus tropicalis Age: 0 Organ: Gastrula Tissue: Xenopus tropicalis Host: DH10B TonA Vector: pCR4-TOPO Vector type: plasmid Insert digest: EcoRI Stop Codon Status: without Description: Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_XGC_XXX has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Xenopus Gene Collection library.