Name: CCSB-DFCI hORFeome collection Library ID: 2538 Organism: Homo sapiens Age: 0 Organ: unspecified Host: DH10B TonA Vector: pDONR223 Vector type: plasmid Insert digest: 5' attB1.1/attB2.1 3' Stop Codon Status: without Description: Full open reading frame clones were created in the Gateway system by the Center for Cancer Systems Biology (CCSB) at DFCI, M. Vidal director, (reference Genome Res. 2004 Oct;14(10B):2128-35 and Genomics 2007 Mar;89(3):307-15) using MGC clones as templates. For PCR amplification, both forward and reverse ORF-specific primers for each MGC clone were designed automatically using the OSP program (Hillier and Green 1991). The forward primer starts from A of the ATG initiation codon, whereas the reverse primer starts from the second nucleotide of the termination codon. The reverse attB2.1 primers do not contain the last nucleotide of the termination codon, so as to allow generation of C-terminal fusion proteins. This library is specifically made of single colonies isolated from the transformed ORF pools. Clones were prepared by the M. Vidal laboratory (Dana Farber Cancer Institute) and generously donated for use by the ORFeome Collaboration.