Name: NCI_CGAP_GCB1 Library ID: 361 Organism: Homo sapiens Age: 0 Stage: adult Organ: B-cell Tissue: flow-sorted tonsils Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was prepared from germinal B-cells (flow-sortedfrom tonsils) provided by Dr. Louis Staudt of the NCI, and was then primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTCATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. This library was previously referred to as Soares NbHTGBC. Note: this is a NCI_CGAP Library.
