Name: Soares 3NME12.5 Library ID: 362 Organism: Mus musculus Strain: C57BL/6 Age: 0 Stage: fetal Organ: embryo Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTATTTTTTTTTTTTTTTTTT 3'], on total mouse RNA [provided by Minoru Ko, Wayne State Univ.]; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
