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cDNA library: Barstead MPL-RB4

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Information for 1 library

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   1. Barstead MPL-RB4

Barstead MPL-RB4

Name: Barstead MPL-RB4
Library ID: 366
Organism: Mus musculus
Strain: FVB/N
Gender: both
Age: 0
Stage: infant
Organ: mixed
Tissue: pooled organs from multiple mice
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGGATCCAAC and GTTGGATTCG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).

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