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cDNA library: Knowles/Solter egg

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Information for 1 library


   1. Knowles/Solter egg

Knowles/Solter egg

Name: Knowles/Solter egg
Library ID: 389
Organism: Mus musculus
Strain: B6D2F1/J
Gender: neither
Age: 0
Stage: embryo
Organ: egg
Tissue: 5000 pooled unfertilized eggs
Host: DH10B
Vector: pBluescribe (modified)
Vector type: phagemid
Insert digest: 5' MluI/SalI 3'
Stop Codon Status: without
Description: Cloned unidirectionally from mRNA prepared from 5000 unfertilized eggs. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.0 kb.

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