Biosciences Directorate logo

I.M.A.G.E. Consortium

“Sharing resources to achieve a common goal — the discovery of all genes”

blue/green horizontal line

cDNA library: Knowles/Solter 8-cell

Home | Site Map | I.Q. search | Libraries by Species | Clones | Vectors | Primers | QC | Arrayed Clones by: Library | Tissue |

Information for 1 library

embryo

   1. Knowles/Solter 8-cell

Knowles/Solter 8-cell

Name: Knowles/Solter 8-cell
Library ID: 391
Organism: Mus musculus
Strain: B6D2F1/J
Age: 0
Stage: embryo
Organ: embryo
Tissue: 2000 pooled 8-cell stage
Host: DH10B
Vector: pSPORT1
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Cloned unidirectionally from mRNA prepared from 2,000 8-cell stage embryos. Primer: NotI(dT): 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Average insert size: 0.7 kb.

horizontal blue line

Home | I.M.A.G.E. | ORFeome | CGAP | MGC | XGC | ZGC | CMLS | Lawrence Livermore | U Iowa | LLNL Disclaimer | HAIB Disclaimer
Web page maintained by
preston.hunter@asu.edu
Email address:
Biological Questions and Comments to
preston.hunter@asu.edu