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cDNA library: Knowles/Solter blastocyst

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Information for 1 library


   1. Knowles/Solter blastocyst

Knowles/Solter blastocyst

Name: Knowles/Solter blastocyst
Library ID: 392
Organism: Mus musculus
Strain: B6D2F1/J
Age: 0
Stage: embryo
Organ: embryo
Tissue: 800 pooled blastocysts
Host: DH10B
Vector: pSPORT1
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: NotI(dT): 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3. Average insert size of combined library is 1 kb.

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