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cDNA library: Gessler Wilms' tumor

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Information for 1 library


   1. Gessler Wilms' tumor

Gessler Wilms' tumor

Name: Gessler Wilms' tumor
Library ID: 440
Organism: Homo sapiens
Age: 0
Stage: adult
Organ: kidney
Tissue: six pooled nephroblastomas; Wilms' tumor
Host: DH10B
Vector: pSPORT1
Vector type: phagemid
Insert digest: 5' SalI/NotI 3'
Stop Codon Status: without
Description: RNA was prepared from a pool of anonymous Wilms' tumors by acid-phenol extraction. cDNA was synthesized with the Life Technologies Superscript plasmid system using an oligo dT-NotI primer for first strand synthesis. A SalI adaptor was ligated to the 5' ends of the clones. cDNA was size- selected on columns, NotI digested, and ligated into NotI/SalI-cut pSPORT1 and electroporated into DH10B cells. Average insert size: 2.0 kb. Library was constructed by Dr. Manfred Gessler.

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