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cDNA library: Barstead MPL-RB7

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Information for 1 library

colon

   1. Barstead MPL-RB7

Barstead MPL-RB7

Name: Barstead MPL-RB7
Library ID: 443
Organism: Mus musculus
Strain: FVB/N
Age: 0
Stage: adult
Organ: colon
Tissue: harvested 72 hours after irradiation with 1400 Gys
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: Tissue obtained from 8 week old mouse. Colon was harvested 72hours after irradiation with 1400 Gys. 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGTCGACATG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).

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