Name: NCI_CGAP_Br1.1 Library ID: 501 Organism: Homo sapiens Gender: female Age: 0 Stage: adult Organ: breast Tissue: pooled bulk tumor Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAACCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is not normalized. (The normalized version of this library is NCI_CGAP_Br2.) Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
