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cDNA library: Soares 1NIB

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Information for 1 library


   1. Soares 1NIB

Soares 1NIB

Name: Soares 1NIB
Library ID: 52
Organism: Homo sapiens
Gender: female
Age: 0
Stage: infant
Organ: brain/CNS
Tissue: whole brain
Host: DH10B
Vector: Lafmid BA
Vector type: phagemid
Insert digest: 5' HindIII/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Hind III adaptors (Pharmacia), digested with Not I and directionally cloned into the Not I and Hind III sites of the Lafmid BA vector. Library went through one round of normalization. Reference: PNAS 91, 9228-9232 (1994) Library constructed by Bento Soares and M. Fatima Bonaldo.

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