Name: NCI_CGAP_Co10 Library ID: 527 Organism: Homo sapiens Age: 0 Stage: adult Organ: colon Tissue: tumor RER+ Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAACGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized. Non-normalized version of this library is named NCI_CGAP_Co9. Library was constructed by Bento Soares and M. Fatima Bonaldo (N-Soares4). Note: this is a NCI_CGAP Library.