Name: Barstead MPL-RB8 Library ID: 563 Organism: Mus musculus Age: 0 Organ: muscle Tissue: undifferentiated tissue culture cell line C2C12 Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGTCGACAAG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation). Source undifferentiated tissue culture cell line C2C12. Library constructed by Bob Barstead. The C2C12 cell line (available from ATCC, catalog # CRL-1772) differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.