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cDNA library: NCI_CGAP_GC4

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Information for 1 library

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   1. NCI_CGAP_GC4

NCI_CGAP_GC4

Name: NCI_CGAP_GC4
Library ID: 634
Organism: Homo sapiens
Age: 0
Stage: adult
Organ: germ cell
Tissue: 3 pooled tumors, including broad spectrum tumor types
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAATCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized. Non-normalized version of this library is named NCI_CGAP_GC3. Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.

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