Name: NCI_CGAP_Kid3 Library ID: 687 Organism: Homo sapiens Age: 0 Stage: adult Organ: kidney Tissue: 2 pooled Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' AACTGGAAGAATTCGCGGCCGCAATGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.