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cDNA library: Soares NFL_T_GBC_S1

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Information for 1 library


   1. Soares NFL_T_GBC_S1

Soares NFL_T_GBC_S1

Name: Soares NFL_T_GBC_S1
Library ID: 689
Organism: Homo sapiens
Age: 0
Organ: mixed
Tissue: pool of lung (19 wks post conception), testis (adult) and flow-sorted tonsils (adult)
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: Equal amounts of plasmid DNA from three normalized libraries(fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo.

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