Name: Sugano mouse embryo mewa Library ID: 721 Organism: Mus musculus Strain: C57BL Age: 0 Stage: embryo Organ: embryo Tissue: whole Host: DH10B Vector: pME18S-FL3 Vector type: phagemid Insert digest: 5' DraIII/DraIII 3' Stop Codon Status: without Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was ligated to a DraIII adaptor [TGTTGGCCTACTGG], digested and cloned into distinct DraIII sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site CACCATGTG). XhoI should be used to isolate the cDNA insert. Size selection was performed to exclude fragments <1.5kb. Library constructed by Dr. Sumio Sugano (University of Tokyo Institute of Medical Science). Custom primers for sequencing: 5' end primer CTTCTGCTCTAAAAGCTGCG and 3' end primer CGACCTGCAGCTCGAGCACA. REFERENCES: Suzuki, Y., Yoshitomo, K., Maruyama, K., Suyama, A., and Sugano, S. Construction and characterization of a full length-enriched and a 5' end enriched cDNA library. Gene 200, 149-156, 1997. Sasaki, Z., Suzuki, Y., Watanabe, M., Imai, J., Shibui, A., Yoshida, K., Hata. H., Yamaguchi, R., Tateyama, S., and Sugano, S. Construction of mouse full length-enriched cDNA libraries by oligo-capping. DNA Research, submitted.