Name: Gatewood hydatidiform mole Library ID: 745 Organism: Homo sapiens Age: 0 Stage: placental Organ: placenta Tissue: hydatidiform mole Host: TOP10 Vector: pCDNAII Vector type: phagemid Insert digest: NotI Stop Codon Status: without Description: Hydatidiform mole is an abnormal human pregnancy which is encoded exclusively by the paternal genome. Complete hydatidiform mole consists of placental tissue only. First stand synthesis was initiated using a polyT-NotI primer. Following addition of BstX1 linkers, the completed cDNA was restricted with NotI. The restricted cDNA was size fractionated on agarose and the portion >2 kb was cloned into the plasmid vector pcDNAII (Invitrogen). The original tranformation mix was frozen in glycerol without amplification and clones were arrayed directly from that glycerol stock. Library prepared by Dr. Joe Gatewood (Los Alamos National Laboratory). REF: "Gene Expression in an Abnormal Human Pregnancy", Joe M. Gatewood, Beckie Lobb, Cheryl Lemanski, Karen Denison, and Virgene Church.