Name: Soares N2b5HB55Y Library ID: 84 Organism: Homo sapiens Age: 0 Stage: adult Organ: brain/CNS Tissue: whole brain Host: DH10B Vector: pT7T3D-PacI Vector type: plasmid Insert digest: 5' EcoRI/NotI 3' Stop Codon Status: without Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot50, and contains inserts ranging from 0.5 to 1.5 kb. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
