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pufferfish (Fugu rubripes) cDNA Library Information

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Information for 15 libraries (by tissue)

brain/CNS

   1. Fugu hgmpF adult brain

eye

   2. Fugu hgmpG adult eye

fin

   3. Fugu UT11 adult fin

gill

   4. Fugu hgmpC adult gill

gonad

   5. Fugu hgmpE adult gonad

gut

   6. Fugu hgmpD adult gut
   7. Fugu UT6 adult gut

kidney

   8. Fugu hgmpJ adult kidney

liver

   9. Fugu hgmpH adult liver

muscle

   10. Fugu UT12 adult muscle

ovary

   11. Fugu UT13 adult ovary

skin

   12. Fugu UT7 adult skin

spleen

   13. Fugu hgmpB adult spleen

whole body

   14. Fugu hgmpA whole adult
   15. Fugu hgmpK whole adult

Fugu hgmpF adult brain

Name: Fugu hgmpF adult brain
Library ID: 1943
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: brain/CNS
Tissue: brain
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult brain. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu hgmpG adult eye

Name: Fugu hgmpG adult eye
Library ID: 1944
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: eye
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult eye. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu UT11 adult fin

Name: Fugu UT11 adult fin
Library ID: 1953
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: fin
Host: DH10B TonA
Vector: pBluescript-FL
Vector type: phagemid
Insert digest: 5' PflmI/PflmI 3'
Stop Codon Status: without
Description: pBluescript-FL is created from pBluescript II phagemid SK(+). Its BamHI-SmaI sites (in the MCS) were converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is destroyed in the process). The rest of the vector is untouched. 1st strand cDNA was primed with an oligo(dT) primer. Double-stranded cDNA was ligated into PflMI adaptors and inserted into the two PflMI sites in the T3 (5') to T7 (3') direction. PflMi can be used to isolate the cDNA insert. Library materials provided by G. Elgar (UK MRC HGMP-RC) and constructed and donated by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi, and S. Watabe (University of Tokyo, Institute of Medical Science and Laboratory of Aquatic Molecular Biology and Biotechnology). T3 should be used for sequencing the 5' end of clones and T7 for the 3' end.

Fugu hgmpC adult gill

Name: Fugu hgmpC adult gill
Library ID: 1940
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: gill
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult gill. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu hgmpE adult gonad

Name: Fugu hgmpE adult gonad
Library ID: 1942
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: gonad
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult gonad. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu hgmpD adult gut

Name: Fugu hgmpD adult gut
Library ID: 1941
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: gut
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult gut. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu UT6 adult gut

Name: Fugu UT6 adult gut
Library ID: 1956
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: gut
Host: DH10B TonA
Vector: pBluescript-FL
Vector type: phagemid
Insert digest: 5' PflmI/PflmI 3'
Stop Codon Status: without
Description: pBluescript-FL is created from pBluescript II phagemid SK(+). Its BamHI-SmaI sites (in the MCS) were converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is destroyed in the process). The rest of the vector is untouched. 1st strand cDNA was primed with an oligo(dT) primer. Double-stranded cDNA was ligated into Pf1MI adaptors and inserted into the two PflMI sites in the T3 (5') to T7 (3') direction. PflMi can be used to isolate the cDNA insert. Library materials provided by G. Elgar (UK MRC HGMP-RC) and constructed and donated by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi, and S. Watabe (University of Tokyo, Institute of Medical Science and Laboratory of Aquatic Molecular Biology and Biotechnology). T3 should be used for sequencing the 5' end of clones and T7 for the 3' end.

Fugu hgmpJ adult kidney

Name: Fugu hgmpJ adult kidney
Library ID: 1946
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: kidney
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult kidney. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu hgmpH adult liver

Name: Fugu hgmpH adult liver
Library ID: 1945
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: liver
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult liver. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu UT12 adult muscle

Name: Fugu UT12 adult muscle
Library ID: 1954
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: muscle
Host: DH10B TonA
Vector: pBluescript-FL
Vector type: phagemid
Insert digest: 5' PflmI/PflmI 3'
Stop Codon Status: without
Description: pBluescript-FL is created from pBluescript II phagemid SK(+). Its BamHI-SmaI sites (in the MCS) were converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is destroyed in the process). The rest of the vector is untouched. 1st strand cDNA was primed with an oligo(dT) primer. Double-stranded cDNA was ligated into PflMI adaptors and inserted into the two PflMI sites in the T3 (5') to T7 (3') direction. PflMi can be used to isolate the cDNA insert. Library materials provided by G. Elgar (UK MRC HGMP-RC) and constructed and donated by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi, and S. Watabe (University of Tokyo, Institute of Medical Science and Laboratory of Aquatic Molecular Biology and Biotechnology). T3 should be used for sequencing the 5' end of clones and T7 for the 3' end.

Fugu UT13 adult ovary

Name: Fugu UT13 adult ovary
Library ID: 1955
Organism: Fugu rubripes
Gender: female
Age: 0
Stage: adult
Organ: ovary
Host: DH10B TonA
Vector: pBluescript-FL
Vector type: phagemid
Insert digest: 5' PflmI/PflmI 3'
Stop Codon Status: without
Description: pBluescript-FL is created from pBluescript II phagemid SK(+). Its BamHI-SmaI sites (in the MCS) were converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is destroyed in the process). The rest of the vector is untouched. 1st strand cDNA was primed with an oligo(dT) primer. Double-stranded cDNA was ligated into PflMI adaptors and inserted into the two PflMI sites in the T3 (5') to T7 (3') direction. PflMi can be used to isolate the cDNA insert. Library materials provided by G. Elgar (UK MRC HGMP-RC) and constructed and donated by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi, and S. Watabe (University of Tokyo, Institute of Medical Science and Laboratory of Aquatic Molecular Biology and Biotechnology). T3 should be used for sequencing the 5' end of clones and T7 for the 3' end.

Fugu UT7 adult skin

Name: Fugu UT7 adult skin
Library ID: 1957
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: skin
Host: DH10B TonA
Vector: pBluescript-FL
Vector type: phagemid
Insert digest: 5' PflmI/PflmI 3'
Stop Codon Status: without
Description: pBluescript-FL is created from pBluescript II phagemid SK(+). Its BamHI-SmaI sites (in the MCS) were converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is destroyed in the process). The rest of the vector is untouched. 1st strand cDNA was primed with an oligo(dT) primer. Double-stranded cDNA was ligated into PflMI adaptors and inserted into the two PflMI sites in the T3 (5') to T7 (3') direction. PflMi can be used to isolate the cDNA insert. Library materials provided by G. Elgar (UK MRC HGMP-RC) and constructed and donated by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi, and S. Watabe (University of Tokyo, Institute of Medical Science and Laboratory of Aquatic Molecular Biology and Biotechnology). T3 should be used for sequencing the 5' end of clones and T7 for the 3' end.

Fugu hgmpB adult spleen

Name: Fugu hgmpB adult spleen
Library ID: 1939
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: spleen
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: adult spleen. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu hgmpA whole adult

Name: Fugu hgmpA whole adult
Library ID: 1938
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: whole body
Tissue: whole fish
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: whole adult fish. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

Fugu hgmpK whole adult

Name: Fugu hgmpK whole adult
Library ID: 1947
Organism: Fugu rubripes
Age: 0
Stage: adult
Organ: whole body
Host: DH10B TonA
Vector: pBluescript II KS+
Vector type: phagemid
Insert digest: 5' EcoRI/XhoI 3'
Stop Codon Status: without
Description: Library is oligo-dT primed and directionally cloned using cDNA prepared with the Stratagene cDNA synthesis kit. Tissue source: whole adult fish. Library materials and construction by Greg Elgar (UK MRC HGMP-RC).

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